Larochelle D A, Vithalani K K, De Lozanne A
Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA.
J Cell Biol. 1996 Jun;133(6):1321-9. doi: 10.1083/jcb.133.6.1321.
Several members of the rho/rac family of small GTP-binding proteins are known to regulate the distribution of the actin cytoskeleton in various subcellular processes. We describe here a novel rac protein, racE, which is specifically required for cytokinesis, an actomyosin-mediated process. The racE gene was isolated in a molecular genetic screen devised to isolate genes required for cytokinesis in Dictyostelium. Phenotypic characterization of racE mutants revealed that racE is not essential for any other cell motility event, including phagocytosis, chemotaxis, capping, or development. Our data provide the first genetic evidence for the essential requirement of a rho-like protein, specifically in cytokinesis, and suggest a role for these proteins in coordinating cytokinesis with the mitotic events of the cell cycle.
已知小GTP结合蛋白的rho/rac家族的几个成员在各种亚细胞过程中调节肌动蛋白细胞骨架的分布。我们在此描述一种新的rac蛋白，即racE，它是胞质分裂（一种肌动球蛋白介导的过程）所特需的。racE基因是在为分离盘基网柄菌胞质分裂所需基因而设计的分子遗传学筛选中分离得到的。racE突变体的表型特征表明，racE对于任何其他细胞运动事件（包括吞噬作用、趋化性、帽化或发育）都不是必需的。我们的数据首次提供了遗传证据，证明一种类rho蛋白在胞质分裂中具有必不可少的需求，并表明这些蛋白在将胞质分裂与细胞周期的有丝分裂事件协调起来方面发挥作用。
