Reifenberger J, Reifenberger G, Ichimura K, Schmidt E E, Wechsler W, Collins V P
Department of Neuropathology, Heinrich-Heine-University, Düsseldorf, Germany.
Am J Pathol. 1996 Jul;149(1):29-35.
A series of 13 oligodendrogliomas (WHO grade II) and 20 anaplastic oligodendrogliomas (WHO grade III) was studied for gene amplification and expression of the epidermal growth factor receptor gene (EGFR). EGFR gene amplification was found in only one case of anaplastic oligodendroglioma, which additionally showed a deletion/rearrangement at the 5' end of the gene. Northern blot analysis, however, revealed increases of EGFR mRNA expression relative to non-neoplastic control brain in 6 of 13 oligodendrogliomas and 10 of 18 anaplastic oligodendrogliomas. All cases with increased mRNA expression showed strong immunoreactivity for EGFR protein. Our findings thus indicate that increased expression of EGFR mRNA and protein is common in low-grade and high-grade oligodendroglial tumors and in the vast majority of cases is not caused by gene amplification.
对13例少突胶质细胞瘤（世界卫生组织二级）和20例间变性少突胶质细胞瘤（世界卫生组织三级）进行了研究，以检测表皮生长因子受体基因（EGFR）的基因扩增和表达情况。仅在1例间变性少突胶质细胞瘤中发现EGFR基因扩增，该病例在基因的5'端还显示出缺失/重排。然而，Northern印迹分析显示，在13例少突胶质细胞瘤中的6例以及18例间变性少突胶质细胞瘤中的10例中，相对于非肿瘤对照脑，EGFR mRNA表达增加。所有mRNA表达增加的病例对EGFR蛋白均表现出强免疫反应性。因此，我们的研究结果表明，EGFR mRNA和蛋白表达增加在低级别和高级别少突胶质细胞瘤中很常见，并且在绝大多数情况下不是由基因扩增引起的。
