Jockusch B M, Bubeck P, Giehl K, Kroemker M, Moschner J, Rothkegel M, Rüdiger M, Schlüter K, Stanke G, Winkler J
Cell Biology, Zoological Institute, Technical University of Braunschweig, Germany.
Annu Rev Cell Dev Biol. 1995;11:379-416. doi: 10.1146/annurev.cb.11.110195.002115.
This article outlines the present knowledge of the architecture, molecular composition, and dynamics of focal contacts of adhesive animal cells. These structures, developed at the plasma membrane at sites where cells touch their substratum, are essential for cellular attachment in tissue formation during embryogenesis and wound healing. In tissue culture, they are particularly prominent and thus amenable to detailed investigation. Focal contacts consist of a cytoplasmic face, comprising cytoskeletal elements, a transmembrane connecting region, and a extracellular face composed of proteins of the extracellular matrix. The molecular anatomy of the numerous proteins involved, the basis for classifying them as structural or regulatory components, and their in vitro interactions are described. Based on this information, current models on the dynamics of their assembly and of possible regulatory mechanisms involving a variety of signal transduction pathways are discussed.
本文概述了目前关于黏附动物细胞黏着斑的结构、分子组成和动力学的知识。这些结构在细胞膜上细胞与底物接触的部位形成，对于胚胎发育和伤口愈合过程中组织形成时的细胞黏附至关重要。在组织培养中，它们尤为突出，因此便于进行详细研究。黏着斑由一个包含细胞骨架成分的胞质面、一个跨膜连接区域和一个由细胞外基质蛋白组成的细胞外面组成。文中描述了众多相关蛋白质的分子结构、将它们分类为结构或调节成分的依据以及它们在体外的相互作用。基于这些信息，讨论了关于其组装动力学以及涉及多种信号转导途径的可能调节机制的当前模型。
