Derényi I, Vicsek T
Department of Atomic Physics, Eötvös University, Budapest, Hungary.
Proc Natl Acad Sci U S A. 1996 Jun 25;93(13):6775-9. doi: 10.1073/pnas.93.13.6775.
Recently individual two-headed kinesin molecules have been studied in in vitro motility assays revealing a number of their peculiar transport properties. In this paper we propose a simple and robust model for the kinesin stepping process with elastically coupled Brownian heads that show all of these properties. The analytic and numerical treatment of our model results in a very good fit to the experimental data and practically has no free parameters. Changing the values of the parameters in the restricted range allowed by the related experimental estimates has almost no effect on the shape of the curves and results mainly in a variation of the zero load velocity that can be directly fitted to the measured data. In addition, the model is consistent with the measured pathway of the kinesin ATPase.
最近，单个双头驱动蛋白分子已在体外运动测定中得到研究，揭示了它们的许多独特运输特性。在本文中，我们提出了一个简单而稳健的驱动蛋白步移过程模型，该模型中弹性耦合的布朗头部展现出了所有这些特性。对我们模型的解析和数值处理与实验数据拟合得非常好，并且实际上没有自由参数。在相关实验估计所允许的受限范围内改变参数值，对曲线形状几乎没有影响，主要导致零负载速度的变化，而这可以直接与测量数据拟合。此外，该模型与驱动蛋白ATP酶的测量路径一致。
