Haraguchi H, Saito T, Ishikawa H, Date H, Kataoka S, Tamura Y, Mizutani K
Faculty of Engineering, Fukuyama University, Japan.
Planta Med. 1996 Jun;62(3):217-21. doi: 10.1055/s-2006-957863.
A biphenyl compound, 3,4,3',4'-tetrahydroxy-5,5'-diisopropyl-2,2'-dimethylbiphenyl (1), and a flavonoid, eriodicytol (2), were isolated as antioxidative components from the leaves of Thymus vulgaris by bioassay-directed fractionation. These compounds inhibited superoxide anion production in the xanthine/xanthine oxidase system. Mitochondrial and microsomal lipid peroxidation induced by Fe(III)-ADP/NADH or Fe(III)-ADP/NADPH were also inhibited by these compounds. Compound 1 is an extremely potent antioxidant; complete inhibition was observed at 1 microM against both microsomal and mitochondrial peroxidation. Furthermore, compound 1 protected red cells against oxidative hemolysis. These phenolic compounds were shown to be effective to protect biological systems against various oxidative stresses.
通过生物活性导向分离法，从百里香的叶子中分离出一种联苯化合物3,4,3',4'-四羟基-5,5'-二异丙基-2,2'-二甲基联苯（1）和一种黄酮类化合物圣草酚（2）作为抗氧化成分。这些化合物在黄嘌呤/黄嘌呤氧化酶系统中抑制超氧阴离子的产生。由Fe(III)-ADP/NADH或Fe(III)-ADP/NADPH诱导的线粒体和微粒体脂质过氧化也受到这些化合物的抑制。化合物1是一种极其有效的抗氧化剂；在1微摩尔浓度下，对微粒体和线粒体过氧化均观察到完全抑制。此外，化合物1保护红细胞免受氧化溶血。这些酚类化合物被证明能有效保护生物系统免受各种氧化应激。
