Haraguchi H, Ishikawa H, Mizutani K, Tamura Y, Kinoshita T
Faculty of Engineering, Fukuyama University, Japan.
Bioorg Med Chem. 1998 Mar;6(3):339-47. doi: 10.1016/s0968-0896(97)10034-7.
Licochalcone A, B, C, D and echinatin, retrochalcones isolated from the roots of Glycyrrhiza inflata (licorice), along with an ordinary chalcone isoliquiritigenin, were assessed for their inhibitory activities on lipid peroxidation in various systems and radical scavenging activity. Among those tested, licochalcones B and D strongly inhibited superoxide anion production in the xanthine/xanthine oxidase system. These two compounds also showed potent scavenging activity on DPPH radical. Microsomal lipid peroxidation induced by Fe(III)-ADP/NAPDH was inhibited almost completely by 3 micrograms/ml of licochalcones B and D. Mitochondrial lipid peroxidation induced by Fe(III)-ADP/NADH was more sensitive to these retrochalcones; almost complete inhibition was observed at 10 micrograms/ml of all retrochalcones tested. Licochalcones B and D scavenged superoxide anion in microsome. Furthermore, these retrochalcones protected red cells against oxidative hemolysis. These phenolic compounds were shown to be effective to protect biological systems against various oxidative stresses.
从胀果甘草（甘草）根部分离得到的甘草查尔酮A、B、C、D和刺芒柄花素（反式查尔酮），以及普通查尔酮异甘草素，对它们在不同体系中的脂质过氧化抑制活性和自由基清除活性进行了评估。在测试的这些物质中，甘草查尔酮B和D强烈抑制黄嘌呤/黄嘌呤氧化酶体系中超氧阴离子的产生。这两种化合物对DPPH自由基也表现出很强的清除活性。3微克/毫升的甘草查尔酮B和D几乎完全抑制了由Fe(III)-ADP/NAPDH诱导的微粒体脂质过氧化。由Fe(III)-ADP/NADH诱导的线粒体脂质过氧化对这些反式查尔酮更敏感；在测试的所有反式查尔酮浓度为10微克/毫升时，观察到几乎完全抑制。甘草查尔酮B和D清除微粒体中的超氧阴离子。此外，这些反式查尔酮保护红细胞免受氧化溶血。这些酚类化合物被证明能有效保护生物体系免受各种氧化应激。
