黄酮类化合物对自由基诱导的红细胞溶血的保护作用。
Protective effect of flavonoids against red blood cell hemolysis by free radicals.
作者信息
Asgary S, Naderi Gh, Askari N
机构信息
Isfahan Cardiovascular Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
出版信息
Exp Clin Cardiol. 2005 Summer;10(2):88-90.
BACKGROUND
Flavonoids are polyphenolic substances with antioxidant properties, and they are found in different vegetables and fruits. Epidemiological studies have shown that the consumption of flavonoids reduces the prevalence of cardiovascular diseases. The use of synthetic antioxidants, however, has been limited because of their toxicity. Therefore, medical researchers have intensified their quest to find natural antioxidants.
OBJECTIVES
To investigate the effect of several pure flavonoids, such as kaempferol, quercetin, morin and rutin, on red blood cell hemolysis and evaluate their -SH capacity as an indicator of membrane protection.
METHODS
The rate of hemolysis and cell membrane -SH capacity were determined by spectrophotometry. Red blood cell peroxidation was induced using 2,2'-azo-bis-(2-amidinopropane) dihydrochloride. The effect of each flavonoid on hemolysis was examined at three concentrations (0.5 mug/mL, 5 mug/mL and 10 mug/mL), however, only the greatest concentration (10 mug/mL) of each flavonoid was used to study the effect on -SH groups.
RESULTS
In all cases, the antioxidant activity was dose-dependent. Rutin showed the highest inhibitory effect on hemolysis among flavonoids (42.5%). The protective effect of kaempferol, rutin and morin against -SH group oxidation measured 7.7%, 23.3% and 26.4%, respectively.
CONCLUSIONS
Results showed that flavonoids and flavonoid-containing plants can be used as natural antioxidants for the treatment and prevention of disease conditions, the pathogenesis of which is mediated by lipid peroxidation.
背景
黄酮类化合物是具有抗氧化特性的多酚类物质，存在于不同的蔬菜和水果中。流行病学研究表明，食用黄酮类化合物可降低心血管疾病的患病率。然而，由于其毒性，合成抗氧化剂的使用受到限制。因此，医学研究人员加大了寻找天然抗氧化剂的力度。
目的
研究山奈酚、槲皮素、桑色素和芦丁等几种纯黄酮类化合物对红细胞溶血的影响，并评估其作为膜保护指标的 -SH 能力。
方法
采用分光光度法测定溶血率和细胞膜 -SH 能力。使用 2,2'-偶氮二（2-脒基丙烷）二盐酸盐诱导红细胞过氧化。在三种浓度（0.5μg/mL、5μg/mL 和 10μg/mL）下检测每种黄酮类化合物对溶血的影响，然而，仅使用每种黄酮类化合物的最高浓度（10μg/mL）来研究其对 -SH 基团的影响。
结果
在所有情况下，抗氧化活性均呈剂量依赖性。芦丁在黄酮类化合物中对溶血的抑制作用最高（42.5%）。山奈酚、芦丁和桑色素对 -SH 基团氧化的保护作用分别为 7.7%、23.3% 和 26.4%。
结论
结果表明，黄酮类化合物和含黄酮类化合物的植物可作为天然抗氧化剂用于治疗和预防由脂质过氧化介导发病机制的疾病。
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