Yamamoto S, Shida T, Okimura T, Otabe K, Honda M, Ashida Y, Furukawa E, Sarikaputi M, Naiki M
Department of Immunology, School of Veterinary Medicine, Azabu University, Kanagawa, Japan.
Vet Q. 1994 Jul;16(2):74-7. doi: 10.1080/01652176.1994.9694422.
The concentrations of C-reactive protein (CRP) in serum from dogs diagnosed as normal by clinical, haematological, and biochemical examination were determined by enzyme-linked immunosorbent assay (ELISA) and slide reversed passive latex agglutination (RPLA), using IgG antibody isolated from rabbit anti-canine CRP serum. The mean value of CRP in 66 normal dogs kept in private households was 8.4 +/- 4.9 micrograms/ml by ELISA and 8.5 +/- 6.3 micrograms/ml by RPLA. Thus, no significant difference was demonstrated between the values obtained by ELISA and RPLA. No significant age and sex-related differences were found in the CRP values. The mean concentration of CRP in 84 6-month-old Beagle dogs kept in kennels by breeders was 6.2 +/- 3.9 micrograms/ml by ELISA and 8.0 +/- 4.0 micrograms/ml by RPLA. Again no significant difference was found. The CRP values determined by ELISA and RPLA were closely correlated (r = 0.913). The serum and plasma concentrations of CRP measured by RPLA were also closely correlated (r = 0.994). This indicates that plasma can be used as well as serum to determine CRP concentrations by RPLA.
采用从兔抗犬C反应蛋白（CRP）血清中分离的IgG抗体，通过酶联免疫吸附测定（ELISA）和玻片反向被动乳胶凝集试验（RPLA），测定经临床、血液学和生化检查诊断为正常的犬血清中CRP的浓度。66只饲养在私人家庭中的正常犬，ELISA法测得CRP的平均值为8.4±4.9微克/毫升，RPLA法测得为8.5±6.3微克/毫升。因此，ELISA法和RPLA法测得的值之间无显著差异。CRP值在年龄和性别上无显著差异。84只由饲养员饲养在犬舍中的6月龄比格犬，ELISA法测得CRP的平均浓度为6.2±3.9微克/毫升，RPLA法测得为8.0±4.0微克/毫升。同样未发现显著差异。ELISA法和RPLA法测定的CRP值密切相关（r = 0.913）。RPLA法测定的血清和血浆CRP浓度也密切相关（r = 0.994）。这表明RPLA法测定CRP浓度时，血浆和血清均可使用。
