Leibfritz D
Institut für Organische Chemie, Universität Bremen, Germany.
Anticancer Res. 1996 May-Jun;16(3B):1317-24.
Multinuclear NMR spectroscopy (1H, 13C, 31P) of biological samples reflects their metabolic composition. The analysis of high field and high resolution spectra of body fluids, cell and biopsy extracts requires no separation of a complex mixture into individual metabolites. Pattern recognition methods can be used to classify normal and pathological samples. Stable isotope enriched precursors are used to follow-up biochemical pathways and metabolic trafficking demonstrated for the lipid biosynthesis. Finally, volume selective in vivo spectroscopy or spectroscopic imaging depicts acute and time dependent metabolic events noninvasively in intact tissue within a few minutes.
生物样品的多核核磁共振波谱（1H、13C、31P）反映了它们的代谢组成。对体液、细胞和活检提取物的高场和高分辨率光谱进行分析时，无需将复杂混合物分离成单个代谢物。模式识别方法可用于对正常和病理样品进行分类。稳定同位素富集前体用于追踪脂质生物合成中显示的生化途径和代谢转运。最后，体积选择性体内光谱或光谱成像可在几分钟内对完整组织中的急性和时间依赖性代谢事件进行无创描绘。
