Walther R, Hippe H, Gottschalk G
Appl Environ Microbiol. 1977 Apr;33(4):955-62. doi: 10.1128/aem.33.4.955-962.1977.
With a medium containing citrate as the carbon and energy source, 10 clostridial strains were isolated from various mud samples. Characterization of these strains revealed that they all belonged to the same species, Clostridium sphenoides. Strains of this organism obtained from culture collections were also able to grow citrate, whereas 15 other clostridial species tested were not. Citrate was fermented by C. sphenoides to acetate, ethanol, carbon dioxide, and hydrogen. Experiments with stereospecifically 14C-labeled citrate indicated that citrate lyase was involved in citrate degradation.
在以柠檬酸盐作为碳源和能源的培养基中，从各种泥浆样本中分离出了10株梭菌菌株。对这些菌株的特性分析表明，它们均属于同一物种——楔形梭菌。从菌种保藏中心获得的该微生物菌株也能够利用柠檬酸盐生长，而测试的其他15种梭菌则不能。楔形梭菌将柠檬酸盐发酵生成乙酸盐、乙醇、二氧化碳和氢气。用立体特异性14C标记的柠檬酸盐进行的实验表明，柠檬酸裂合酶参与了柠檬酸盐的降解。
