Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aoba 6-6-11, Aramaki, Aoba-Ku, Sendai, Miyagi, 980-8579, Japan.
Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Oiwake-Cho, Kitashirakawa, Sakyo-Ku, Kyoto, 606-8502, Japan.
Curr Microbiol. 2023 Apr 19;80(5):187. doi: 10.1007/s00284-023-03285-1.
Strain C5-48, an anaerobic intestinal bacterium that potentially accumulates acetaldehyde at levels exceeding its minimum mutagenic concentration (50 µM) in the colon and rectum, was isolated from the feces of a patient with alcoholism. The 16S rRNA gene sequence of strain C5-48 showed high similarity to the corresponding sequences of Lachnoclostridium edouardi Marseille-P3397 (95.7%) and Clostridium fessum SNUG30386 (94.7%). However, phylogenetic analysis using the sequences of the 16S rRNA, rpoB, and hsp60 genes and whole-genome analysis strongly suggested that C5-48 should be included in the genus Enterocloster. The novelty of strain C5-48 was further confirmed by comprehensive average nucleotide identity (ANI) calculations based on its whole-genome sequence, which showed appreciable ANI values with known Enterocloster species (e.g., 74.3% and 73.4% with Enterocloster bolteae WAL 16351 and Enterocloster clostridioformis ATCC 25537, respectively). The temperature range for growth of strain C5-48 was 15-37 °C with an optimum of 37 °C. The pH range for growth was 5.5-10.5 with an optimum of 7.5. The major constituents of the cell membrane lipids of strain C5-48 were 16:0, 14:0, and 18:1 ω7c dimethyl acetal fatty acids. On the basis of the genotypic and phenotypic properties, Enterocloster alcoholdehydrogenati sp. nov. is proposed, with the type strain C5-48 (= JCM 33305 = DSM 109474).
菌株 C5-48 是一种厌氧肠道细菌，它在结肠和直肠中可能积累超过其最低致突变浓度（50µM）的乙醛。该菌株从一名酒精中毒患者的粪便中分离得到。菌株 C5-48 的 16S rRNA 基因序列与 Lachnoclostridium edouardi Marseille-P3397（95.7%）和 Clostridium fessum SNUG30386（94.7%）的相应序列高度相似。然而，基于 16S rRNA、rpoB 和 hsp60 基因序列和全基因组分析的系统发育分析强烈表明，C5-48 应归入 Enterocloster 属。基于全基因组序列的综合平均核苷酸同一性（ANI）计算进一步证实了菌株 C5-48 的新颖性，其与已知的 Enterocloster 种（例如，与 Enterocloster bolteae WAL 16351 和 Enterocloster clostridioformis ATCC 25537 的 ANI 值分别为 74.3%和 73.4%）具有相当高的 ANI 值。菌株 C5-48 的生长温度范围为 15-37°C，最适温度为 37°C。生长的 pH 值范围为 5.5-10.5，最适 pH 值为 7.5。菌株 C5-48 细胞膜脂的主要成分是 16:0、14:0 和 18:1 ω7c 二甲基缩醛脂肪酸。基于基因型和表型特性，建议将 Enterocloster alcoholdehydrogenati sp. nov. 作为新种提出，其模式菌株为 C5-48（= JCM 33305 = DSM 109474）。
