Markovic P, Roenneberg T, Morse D
Institut de Recherche en Biologie Végétale, Université de Montréal, Canada.
J Biol Rhythms. 1996 Mar;11(1):57-67. doi: 10.1177/074873049601100106.
The synthesis rates of 13 individual proteins in Gonyaulax polyedra, resolved by two-dimensional gel electrophoresis, were estimated from the amount of 35S methionine incorporated during in vivo pulse labeling at 2-h intervals over one circadian period. The synthesis rates of three proteins, taken as controls, varied 2-3 fold, and no systematic pattern to these variations was apparent. In contrast, the synthesis rates of 10 other proteins varied at least tenfold and in a smooth and systematic pattern. The patterns of protein synthesis were placed into three different groups, the first occurring during the late day/early night phase, the second during the middle of the night phase, and the third during the late night/early day phase. The length of time that individual proteins within each group could incorporate radiolabel was variable, raising the possibility that additional groups might be present. However, both a replicate experiment in continuous light and a light:dark experiment confirmed the presence of at least three different groups of protein synthesis patterns. Unlike the circadian changes in the synthesis rate of the luciferin binding protein, which produces variations in protein levels that correlate with the bioluminescence rhythm, no substantial changes were found in the levels of any other rhythmically synthesized proteins examined.
通过二维凝胶电泳分离出多甲藻（Gonyaulax polyedra）中13种蛋白质的合成速率，其是根据在一个昼夜周期内每隔2小时进行体内脉冲标记时掺入的35S甲硫氨酸的量来估算的。作为对照的三种蛋白质的合成速率变化了2 - 3倍，且这些变化没有明显的系统模式。相比之下，其他10种蛋白质的合成速率变化至少10倍，且呈平滑且有规律的模式。蛋白质合成模式被分为三个不同的组，第一组出现在傍晚/深夜阶段，第二组出现在午夜阶段，第三组出现在深夜/清晨阶段。每组中单个蛋白质能够掺入放射性标记的时间长度各不相同，这增加了可能存在其他组的可能性。然而，在持续光照下的重复实验以及光照：黑暗实验都证实了至少存在三种不同的蛋白质合成模式组。与荧光素结合蛋白合成速率的昼夜变化不同，荧光素结合蛋白合成速率的变化会导致蛋白质水平的变化与生物发光节律相关，在所检测的任何其他节律性合成蛋白质的水平上均未发现实质性变化。
