多甲藻属生物发光的昼夜节律调节涉及翻译控制。

Morse D, Milos P M, Roux E, Hastings J W

Department of Cellular and Developmental Biology, Biological Laboratories, Harvard University, Cambridge, MA 02138.

Proc Natl Acad Sci U S A. 1989 Jan;86(1):172-6. doi: 10.1073/pnas.86.1.172.

A 10-fold circadian variation in the amount of luciferin binding protein (LBP) in the marine dinoflagellate Gonyaulax polyedra is reported. This protein binds and stabilizes luciferin, the bioluminescence substrate. In early night phase, when bioluminescence is increasing and LBP levels are rising in the cell, pulse labeling experiments show that LBP is being rapidly synthesized in vivo. At other times, the rate of LBP synthesis is at least 50 times lower, while the rate of synthesis of most other proteins remains the same. The LBP mRNA levels, as determined by in vitro translations and by RNA (Northern) hybridizations, do not vary over the daily cycle, indicating that circadian control of bioluminescence in this species is mediated by translation.

据报道，海洋双鞭毛藻多甲藻（Gonyaulax polyedra）中虫荧光素结合蛋白（LBP）的含量存在10倍的昼夜节律变化。这种蛋白质能结合并稳定生物发光底物虫荧光素。在夜晚早期，当生物发光增强且细胞内LBP水平上升时，脉冲标记实验表明LBP在体内快速合成。在其他时间，LBP的合成速率至少低50倍，而大多数其他蛋白质的合成速率保持不变。通过体外翻译和RNA（Northern）杂交测定的LBP mRNA水平在每日周期中没有变化，这表明该物种生物发光的昼夜节律控制是由翻译介导的。