Munk M E, Elser C, Kaufmann S H
Department of Immunology, University of Ulm, Germany.
Immunology. 1996 Feb;87(2):230-5. doi: 10.1046/j.1365-2567.1996.470549.x.
Listeria monocytogenes is a facultative intracellular pathogen that replicates inside mononuclear phagocytes and induces specific cellular immunity. Listeriosis encompasses many clinical syndromes and meningitis is the most frequent clinical manifestation. Human alpha/beta and gamma/delta T cells have been shown to respond to L. monocytogenes antigens and to play an important role in resistance against listerial infection. We investigated the nature of listerial ligands and the influence of the major virulence factor, listeriolysin (hly), on the stimulation of human gamma/delta T cells from healthy individuals. We found that a listerial somatic protein ligand, which is sensitive to proteinase treatment, stimulated gamma/delta T cells in vitro; the majority of Listeria-responsive gamma/delta T cells expressed V gamma 9V delta 2 T-cell receptor chains and human leucocyte antigen-DR molecules; gamma/delta T-cell responses to hly+ and hly- Listeria strains were comparable; L. monocytogenes strains of different virulence stimulated gamma/delta T cells equally. Thus, protein components of L. monocytogenes unrelated to virulence activate human gamma/delta T cells in vitro.
单核细胞增生李斯特菌是一种兼性细胞内病原体，可在单核吞噬细胞内复制并诱导特异性细胞免疫。李斯特菌病包括多种临床综合征，脑膜炎是最常见的临床表现。已证明人类α/β和γ/δ T细胞对单核细胞增生李斯特菌抗原产生反应，并在抵抗李斯特菌感染中发挥重要作用。我们研究了李斯特菌配体的性质以及主要毒力因子李斯特菌溶血素（hly）对健康个体人类γ/δ T细胞刺激的影响。我们发现，一种对蛋白酶处理敏感的李斯特菌体细胞蛋白配体在体外刺激γ/δ T细胞；大多数对李斯特菌有反应的γ/δ T细胞表达Vγ9Vδ2 T细胞受体链和人类白细胞抗原-DR分子；γ/δ T细胞对hly+和hly-李斯特菌菌株的反应相当；不同毒力的单核细胞增生李斯特菌菌株对γ/δ T细胞的刺激相同。因此，单核细胞增生李斯特菌与毒力无关的蛋白质成分在体外激活人类γ/δ T细胞。
