产志贺样毒素大肠杆菌的耐酸性表型及rpoS等位基因的特征分析
Characterization of the acid resistance phenotype and rpoS alleles of shiga-like toxin-producing Escherichia coli.
作者信息
Waterman S R, Small P L
机构信息
Microscopy Branch, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840 USA.
出版信息
Infect Immun. 1996 Jul;64(7):2808-11. doi: 10.1128/iai.64.7.2808-2811.1996.
Abstract
Shiga-like toxin-producing Escherichia coli (SLTEC) strains are an important group of enteric pathogens. In this study we have examined the abilities of 58 SLTEC isolates to survive at pH 2.5 and found 13 of these isolates to be defective in acid resistance. Introduction of rpoS on a plasmid conferred acid resistance to the majority of the acid-sensitive isolates. The rpoS genes from two of these isolates were sequenced; both isolates contained lesions in the rpoS gene resulting in a nonfunctional RpoS. These results show that mutant rpoS alleles exist in natural populations of E. coli. Such mutations may play an important role in determining the infective dose of SLTEC and suggest that isolates may vary in infectivity.
摘要
产志贺样毒素大肠杆菌（SLTEC）菌株是一类重要的肠道病原体。在本研究中，我们检测了58株SLTEC分离株在pH 2.5环境下的存活能力，发现其中13株分离株存在耐酸性缺陷。将rpoS基因导入质粒可使大多数酸敏感分离株获得耐酸性。对其中两株分离株的rpoS基因进行了测序；这两株分离株的rpoS基因均存在损伤，导致RpoS功能丧失。这些结果表明，突变的rpoS等位基因存在于大肠杆菌的自然群体中。此类突变可能在决定SLTEC的感染剂量方面发挥重要作用，并提示分离株的传染性可能存在差异。
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本文引用的文献
1
Acid resistance in enteric bacteria.肠道细菌中的耐酸性
Infect Immun. 1993 Jan;61(1):364-7. doi: 10.1128/iai.61.1.364-367.1993.
2
Trehalose metabolism in Escherichia coli: stress protection and stress regulation of gene expression.大肠杆菌中的海藻糖代谢：应激保护及基因表达的应激调控
Mol Microbiol. 1993 Apr;8(2):205-10. doi: 10.1111/j.1365-2958.1993.tb01564.x.
3
Shiga-like toxin-producing Escherichia coli in Seattle children: a prospective study.西雅图儿童中产志贺毒素大肠杆菌的前瞻性研究。
Gastroenterology. 1993 Dec;105(6):1724-31. doi: 10.1016/0016-5085(93)91069-t.
4
Occurrence of Shiga-like toxin-producing Escherichia coli in retail fresh seafood, beef, lamb, pork, and poultry from grocery stores in Seattle, Washington.华盛顿州西雅图市杂货店零售的新鲜海鲜、牛肉、羊肉、猪肉和家禽中产生志贺样毒素的大肠杆菌的发生率。
Appl Environ Microbiol. 1994 Mar;60(3):1038-40. doi: 10.1128/aem.60.3.1038-1040.1994.
5
Acid and base resistance in Escherichia coli and Shigella flexneri: role of rpoS and growth pH.大肠杆菌和福氏志贺氏菌中的耐酸和耐碱能力：rpoS和生长pH值的作用
J Bacteriol. 1994 Mar;176(6):1729-37. doi: 10.1128/jb.176.6.1729-1737.1994.
6
A swimming-associated outbreak of hemorrhagic colitis caused by Escherichia coli O157:H7 and Shigella sonnei.由大肠杆菌O157:H7和宋内志贺菌引起的与游泳相关的出血性结肠炎暴发。
N Engl J Med. 1994 Sep 1;331(9):579-84. doi: 10.1056/NEJM199409013310904.
7
8
Acid tolerance of enterohemorrhagic Escherichia coli.肠出血性大肠杆菌的耐酸性
Appl Environ Microbiol. 1995 Apr;61(4):1669-72. doi: 10.1128/aem.61.4.1669-1672.1995.
9
Escherichia coli O157:H7: clinical, diagnostic, and epidemiological aspects of human infection.大肠杆菌O157:H7：人类感染的临床、诊断及流行病学方面
Clin Infect Dis. 1995 Jan;20(1):1-8; quiz 9-10. doi: 10.1093/clinids/20.1.1.
10
Microbial competition: Escherichia coli mutants that take over stationary phase cultures.微生物竞争：接管稳定期培养物的大肠杆菌突变体。
Science. 1993 Mar 19;259(5102):1757-60. doi: 10.1126/science.7681219.
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