Zárate V, Belda F
Departamento de Microbiología y Biología Celular, Facultad de Farmacia, Universidad de La Laguna, Tenerife, Spain.
J Appl Bacteriol. 1996 Jan;80(1):45-52. doi: 10.1111/j.1365-2672.1996.tb03188.x.
In order to gain information on the ability of Schizosaccharomyces pombe to process heterologous glycoproteins, the heterologous invertase, obtained from the expression in Schiz. pombe of the SUC2 gene of Saccharomyces cerevisiae, was characterized. In Schiz. pombe the heterologous invertase is secreted into the cell wall and seems to be firmly bound to this structure. After the isolation of the heterologous invertase the study of its enzymatic characteristics revealed that it is more similar to the Sacch. cerevisiae external invertase than to the Schiz. pombe invertase. However, it is glycosylated like the Schiz. pombe invertase since it reacts with the lectin from Bandeiraea simplicifolia seeds conjugated to fluorescein isothiocyanate, which indicates the presence of terminal galactose residues in the enzyme. Moreover, the presence of galactose in the heterologous invertase has been confirmed after analysis of the sugars present in its carbohydrate moiety by gas liquid chromatography.
为了获取粟酒裂殖酵母处理异源糖蛋白能力的相关信息，对从酿酒酵母SUC2基因在粟酒裂殖酵母中表达所获得的异源转化酶进行了表征。在粟酒裂殖酵母中，异源转化酶分泌到细胞壁中，并且似乎与该结构紧密结合。分离出异源转化酶后，对其酶学特性的研究表明，它与酿酒酵母的胞外转化酶比与粟酒裂殖酵母的转化酶更为相似。然而，它与粟酒裂殖酵母的转化酶一样进行了糖基化，因为它能与异硫氰酸荧光素偶联的单叶豆种子凝集素发生反应，这表明该酶中存在末端半乳糖残基。此外，通过气相色谱分析其碳水化合物部分中存在的糖类后，证实了异源转化酶中存在半乳糖。
