Buisman A M, Langermans J A, van Furth R
Department of Infectious Diseases, University Hospital, Leiden, Netherlands.
J Infect Dis. 1996 Aug;174(2):417-21. doi: 10.1093/infdis/174.2.417.
This study assesses the effect of granulocyte colony-stimulating factor (G-CSF) on the outcome of infection with Listeria monocytogenes or Staphylococcus aureus in mice during leukocytopenia induced by sublethal total body irradiation or cyclophosphamide treatment. The role of granulocytes during infection was assessed in the thigh muscle, spleen, and liver. G-CSF treatment in naive mice increased the number of blood granulocytes; upon infection, these numbers increased further, but G-CSF did not affect the outgrowth of bacteria in the tissues. Cyclophosphamide treatment and sublethal irradiation decreased the number of blood granulocytes, which was not affected by G-CSF treatment. However, during infection in cyclophosphamide-treated mice, G-CSF increased the number of granulocytes in the circulation and at the site of infection and decreased the number of bacteria in the tissues. Treatment with G-CSF did not affect the number of granulocytes or the course of infection in irradiated mice.
本研究评估了粒细胞集落刺激因子（G-CSF）对在亚致死性全身照射或环磷酰胺治疗诱导的白细胞减少期间，小鼠感染单核细胞增生李斯特菌或金黄色葡萄球菌的结局的影响。在大腿肌肉、脾脏和肝脏中评估了粒细胞在感染过程中的作用。在未感染的小鼠中，G-CSF治疗增加了血液中粒细胞的数量；感染后，这些数量进一步增加，但G-CSF并未影响组织中细菌的生长。环磷酰胺治疗和亚致死性照射减少了血液中粒细胞的数量，而G-CSF治疗对此没有影响。然而，在环磷酰胺治疗的小鼠感染期间，G-CSF增加了循环中和感染部位的粒细胞数量，并减少了组织中的细菌数量。G-CSF治疗对受照射小鼠的粒细胞数量或感染进程没有影响。
