空腹大鼠的葡萄糖周转率及氟烷麻醉的影响。

Glucose turnover in the post-absorptive rat and the effects of halothane anaesthesia.

作者信息

Heath D F, Frayn K N, Rose J G

出版信息

Biochem J. 1977 Mar 15;162(3):653-7. doi: 10.1042/bj1620653.

DOI:10.1042/bj1620653

PMID:869909

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1164649/

Abstract

Rates and rate coefficients of glucose utilization and replacement in post-absorptive rats, either conscious or under halothane anaesthesia, were determined in a thermoneutral environment by using [5-3H]- and [U-14C]glucose. Label was not injected into rats under halothane until about 0.5h after anaesthesia was initiated. 2. Comparison with the results for 24h-starved rats in the preceding paper [Heath et al. (1977) Biochem. J. 162, 643-651] showed that insulin concentrations were considerably higher but rate coefficients for glucose utilization were little altered in post-absorptive rats. Sensitivity to insulin was thus considerably increased by a 24h period of starvation in the rat. 3. Fractional recycling of glucose carbon in post-absorptive rats was under one-half of that in starved rats, reflecting the larger contribution of liver glycogenolysis to glucose production in the former. 4. In post-absorptive rats halothane decreased the mean rate of glucose utilization by about 17%. This decrease was associated with an increase in mean plasma insulin concentration, showing that halothane decreased sensitivity to insulin. 5. Recycling was slightly increased by halothane, indicating that the contribution of liver glycogen to the total glucogenic rate was decreased, probably because liver glycogen concentration were about 40% lower throughout the rate determinations in halothane. 6. Comparison of our results with earlier work shows that during and shortly after induction of halothane anaesthesia glucose turnover must have been greatly increased whereas from about 0.5h after induction it was decreased.

摘要

在热中性环境中，使用[5-³H]-和[U-¹⁴C]葡萄糖测定了清醒或氟烷麻醉下的禁食大鼠葡萄糖利用和补充的速率及速率系数。在开始麻醉约0.5小时后才给氟烷麻醉下的大鼠注射标记物。2. 与前文[希思等人（1977年），《生物化学杂志》162卷，643 - 651页]中24小时饥饿大鼠的结果比较表明，禁食大鼠的胰岛素浓度显著更高，但葡萄糖利用的速率系数变化不大。因此，大鼠禁食24小时后对胰岛素的敏感性显著增加。3. 禁食大鼠中葡萄糖碳的部分再循环不到饥饿大鼠的一半，这反映出肝脏糖原分解对前者葡萄糖生成的贡献更大。4. 在禁食大鼠中，氟烷使葡萄糖利用的平均速率降低了约17%。这种降低与平均血浆胰岛素浓度的增加有关，表明氟烷降低了对胰岛素的敏感性。5. 氟烷使再循环略有增加，这表明肝脏糖原对总糖异生速率的贡献降低，可能是因为在整个氟烷麻醉期间的速率测定中肝脏糖原浓度降低了约40%。6. 将我们的结果与早期研究进行比较表明，在氟烷麻醉诱导期间及之后不久，葡萄糖周转率必定大幅增加，而在诱导后约0.5小时起则降低。