Ogrydziak D M, Demain A L, Tannenbaum S R
Biochim Biophys Acta. 1977 Apr 27;497(2):525-38. doi: 10.1016/0304-4165(77)90209-4.
Production of extracellular protease by Candida lipolytica NRRL Y-1094 was derepressed upon transfer to carbon-, nitrogen- or sulphur-free medium but not upon transfer to phosphorus-free medium. The protease activities produced under the three nutrient limitations had alkaline pH optima and similar substrate and inhibitor specificities. Any one of the following three conditions was found to be sufficient for derepression of extracellular protease: (a) "poor" carbon source, (b) cysteine intracellular pool below 0.5 micronmol/g dry weight cells and (c) ammonia intracellular pool below 10 micronmol/g dry weight cells. Thus, extracellular protease production in C. lipolytica was subject to at least three different regulatory controls, carbon, sulphur and nitrogen repression. Intracellular cysteine and ammonia appeared to be the metabolic signals for sulphur and nitrogen repression, respectively. Anabolic glutamate dehydrogenase did not act as a regulatory protein mediating nitrogen repression. Exogenous protein had an inductive effect on extracellular protease production.
解脂耶氏酵母NRRL Y - 1094在转接到无碳、无氮或无硫培养基上时，细胞外蛋白酶的产生被解除阻遏，但转接到无磷培养基上时则不然。在三种营养限制条件下产生的蛋白酶活性具有碱性pH最适值，且底物和抑制剂特异性相似。发现以下三种条件中的任何一种都足以解除细胞外蛋白酶的阻遏：(a) “劣质”碳源，(b) 细胞内半胱氨酸池低于0.5微摩尔/克干重细胞，以及(c) 细胞内氨池低于10微摩尔/克干重细胞。因此，解脂耶氏酵母中细胞外蛋白酶的产生受到至少三种不同的调控控制，即碳、硫和氮阻遏。细胞内半胱氨酸和氨似乎分别是硫和氮阻遏的代谢信号。合成代谢型谷氨酸脱氢酶不作为介导氮阻遏的调控蛋白。外源蛋白对细胞外蛋白酶的产生有诱导作用。
