Grace D M
Can J Surg. 1977 May;20(3):249-54.
Spontaneous mammary adenocarcinoma was implanted subcutaneously into the flank or leg of inbred C3H/HeJ mice. Excision or cryotherapy of flank tumours was followed by cure in 47 and 52% respectively. Amputation or cryotherapy of tumours of the hind leg resulted in a cure in 70% for each method. Rechallenge of surviving mice resulted in initial delay of growth in mice treated by cryotherapy, but final host acceptance and survival were the same for each method of treatment. The tumour was grown in tissue culture; significant antibody levels after cryotherapy could not be detected by a mixed antiglobulin technique. It is concluded that cryotherapy can destroy the tumour, but this method offers little immunologic advance for weakly antigenic tumours. Careful monitoring of temperature is essential for successful cryotherapy. In the same series of experiments barbiturate anesthesia and tumour exploration did not alter tumour growth or mortality.
将自发性乳腺腺癌皮下植入近交系C3H/HeJ小鼠的胁腹或腿部。切除或冷冻治疗胁腹肿瘤后，治愈率分别为47%和52%。对后腿肿瘤进行截肢或冷冻治疗，每种方法的治愈率均为70%。对存活小鼠进行再次攻击，冷冻治疗的小鼠肿瘤生长初期延迟，但每种治疗方法最终宿主的接受情况和存活率相同。肿瘤在组织培养中生长；通过混合抗球蛋白技术未检测到冷冻治疗后有显著的抗体水平。结论是，冷冻疗法可以破坏肿瘤，但对于弱抗原性肿瘤，这种方法在免疫方面进展甚微。成功进行冷冻治疗必须仔细监测温度。在同一系列实验中，巴比妥类麻醉和肿瘤探查并未改变肿瘤生长或死亡率。
