York B, Lei K, West D B
Obesity, Diabetes and Metabolism Section, Pennington Biomedical Research Center, 6400 Perkins Road, Louisiana State University, Baton Rouge, Louisiana 70808, USA.
Mamm Genome. 1996 Sep;7(9):677-81. doi: 10.1007/s003359900204.
Details of a new model of diet-dependent polygenic obesity are presented. CAST/Ei (Mus m. castaneus) mice remain lean after 12 weeks on a high-fat (32 kcal% fat) diet, while C57BL/6J mice become obese. The genes responsible for the obesity segregate in an F2 population derived from an intercross between CAST/Ei and C57BL/6J mice. Quantitative trait analysis, with simple sequence length polymorphisms (SSLPs) at loci previously linked to rodent obesities, identified a quantitative trait locus (QTL) on Chromosome (Chr) 15, accounting for approximately 9% of the variance in adiposity and 14% of the variance in mesenteric depot size. This locus appears to be at the same location as the dietary obesity-3 (Do3) locus controlling body fat content, which was previously identified in an F2 population derived from an SWR/J x AKR/J cross. This is also at the same location as the multigenic obesity-4 (Mob4) locus found in BSB mice, which display spontaneous polygenic obesity. Suggestive linkage also was found at loci close to the single gene mutations Ay on Chr 2 and tub on Chr 7.
本文介绍了一种新型饮食依赖性多基因肥胖模型的详细信息。CAST/Ei（小家鼠栗色亚种）小鼠在高脂（32千卡%脂肪）饮食喂养12周后仍保持瘦体重，而C57BL/6J小鼠则会肥胖。导致肥胖的基因在CAST/Ei和C57BL/6J小鼠杂交产生的F2群体中分离。利用先前与啮齿动物肥胖相关位点的简单序列长度多态性（SSLPs）进行数量性状分析，在15号染色体（Chr）上鉴定出一个数量性状位点（QTL），该位点约占肥胖度变异的9%和肠系膜脂肪库大小变异的14%。该位点似乎与之前在SWR/J×AKR/J杂交产生的F2群体中鉴定出的控制体脂含量的饮食性肥胖-3（Do3）位点位于同一位置。这也与在表现出自发性多基因肥胖的BSB小鼠中发现的多基因肥胖-4（Mob4）位点位于同一位置。在靠近2号染色体上的单基因突变Ay和7号染色体上的tub的位点也发现了提示性连锁。
