Chaudhary S, Crossland L
CIBA Agricultural Biotechnology Research Unit, Research Triangle Park, NC 27709, USA.
Plant Mol Biol. 1996 Mar;30(6):1247-57. doi: 10.1007/BF00019556.
Trg-31 is a pea gene which is rapidly induced in response to dehydration stress. Trg-31 belongs to a family of membrane intrinsic proteins that play a role in facilitating inter-membrane transport. In order to understand the mechanism of regulation of Trg-31 expression we defined the cis-acting elements of the Trg-31 promoter and the trans-acting factors that interact with them. Deletion analysis of the Trg-31 promoter in transgenic tobacco plants indicated the presence of two positive regulatory region: (1) between the -1412 to -1066 nucleotides and (2) between the -559 to -391 nucleotides, with respect to the transcription initiation site. A negative regulatory element was also identified between -1066 and -559 nucleotides. All three regulatory regions specifically regulated Trg-31 induction in leaf tissue and had little or no effect on Trg-31 induction in root tissue. Gel-mobility shift assays indicated the presence of leaf nuclear proteins that interacted with the -1412 to -1066 and -1066 to -559 regions and an 'ABRE-like' sequence at -74 in the 5' promoter region of Trg-31.
Trg-31是一种豌豆基因，在脱水胁迫下会迅速被诱导。Trg-31属于膜内在蛋白家族，在促进膜间运输中发挥作用。为了了解Trg-31表达的调控机制，我们确定了Trg-31启动子的顺式作用元件以及与之相互作用的反式作用因子。对转基因烟草植株中Trg-31启动子的缺失分析表明，存在两个正向调控区域：（1）相对于转录起始位点，在-1412至-1066核苷酸之间；（2）在-559至-391核苷酸之间。在-1066和-559核苷酸之间也鉴定出一个负向调控元件。所有这三个调控区域都特异性地调节叶片组织中Trg-31的诱导，而对根组织中Trg-31的诱导几乎没有影响。凝胶迁移率变动分析表明，存在与Trg-31 5'启动子区域中-1412至-1066和-1066至-559区域以及-74处的“类ABRE”序列相互作用的叶片核蛋白。
