辛德毕斯病毒的感染性病毒-抗体复合物
Infectious virus-antibody complexes of sindbis virus.
作者信息
Symington J, McCann A K, Schlesinger M J
出版信息
Infect Immun. 1977 Mar;15(3):720-5. doi: 10.1128/iai.15.3.720-725.1977.
Abstract
Infectious virus-antibody complexes were formed when Sindbis virus was reacted with antibodies raised against purified viral envelope glycoproteins E1 and E2 as well as against preparations of intact virus. Results from rate zonal centrifugation in sucrose gradients of the complex formed with anti-E1 sera showed this complex to be about the same size as virions. A test of virus neutralization, based on direct plaque assay, by antibodies raised in rabbits and mice given virus in complete Freund adjuvant indicated the presence of antibodies able to complex but not neutralize virus. Conditions were found in which most of the virus was complexed and protected fron neutralization, suggesting that these sera may contain a mixed population of antiviral antibodies with different specificities and different avidities.
摘要
当辛德毕斯病毒与针对纯化病毒包膜糖蛋白E1和E2以及完整病毒制剂产生的抗体反应时，形成了感染性病毒-抗体复合物。用抗E1血清形成的复合物在蔗糖梯度中进行速率区带离心的结果表明，该复合物的大小与病毒粒子大致相同。基于直接空斑试验的病毒中和试验表明，在完全弗氏佐剂中给兔子和小鼠接种病毒后产生的抗体能够与病毒形成复合物，但不能中和病毒。发现了这样的条件，即大多数病毒形成了复合物并免受中和作用，这表明这些血清可能含有具有不同特异性和不同亲和力的抗病毒抗体混合群体。
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