结构蛋白掺入辛德毕斯病毒粒子的动力学
Kinetics of incorporation of structural proteins into Sindbis virions.
作者信息
Scheele C M, Pfefferkorn E R
出版信息
J Virol. 1969 Apr;3(4):369-75. doi: 10.1128/JVI.3.4.369-375.1969.
Abstract
The morphogenesis of Sindbis virus was studied by determining the kinetics with which newly synthesized nucleocapsid and envelope proteins appeared in virions released into the extracellular medium. Assembly of the nucleocapsid was more rapid than modification of the cellular membrane by the addition of the viral envelope protein. However, both viral structural proteins were efficiently incorporated into virions; a 0.5-hr pulse-labeling period resulted in the release of maximally labeled virus during the next hour. When protein synthesis was inhibited, release of virus soon declined even though large amounts of both viral structural proteins were present within the cell and ribonucleic acid replication was unaffected.
摘要
通过测定新合成的核衣壳蛋白和包膜蛋白出现在释放到细胞外培养基中的病毒粒子中的动力学，研究了辛德毕斯病毒的形态发生。核衣壳的组装比通过添加病毒包膜蛋白对细胞膜进行修饰要快。然而，两种病毒结构蛋白都能有效地整合到病毒粒子中；0.5小时的脉冲标记期导致在接下来的一小时内释放出标记程度最高的病毒。当蛋白质合成受到抑制时，即使细胞内存在大量的两种病毒结构蛋白且核糖核酸复制未受影响，病毒的释放也很快下降。
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