Nogawa M, Kageyama T, Nakatani A, Taguchi G, Shimosaka M, Okazaki M
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Nagano, Japan.
Biosci Biotechnol Biochem. 1996 May;60(5):784-8. doi: 10.1271/bbb.60.784.
A mycovirus (named FusoV) from the phytopathogenic fungus, Fusarium solani f. sp. robiniae SUF704, has two kinds of double-stranded (ds) RNA genomes, designated M1 and M2. The cDNAs were constructed from FusoV genomic dsRNAs. The sequences of M1 and M2 cDNAs comprised 1645 and 1445bp, respectively. Sequence analysis showed that each dsRNA had a single long open reading frame (ORF) on only one of the strands. M1 ORF encodes a 519-amino acid residue polypeptide with a predicted molecular mass of 60 kDa. RNA-dependent RNA polymerase-conserved motifs were identified in the predicted amino acid sequence, and the polymerase synthesized dsRNA in vitro. The M2 ORF encodes a polypeptide of 413 amino acid residues with a predicted molecular mass of 44 kDa. The predicted amino acid sequence contained the sequence corresponding to those found in the purified 44-kDa capsid protein of FusoV.
来自植物病原真菌茄腐镰刀菌（Fusarium solani f. sp. robiniae）SUF704的一种真菌病毒（命名为FusoV）有两种双链（ds）RNA基因组，分别命名为M1和M2。FusoV基因组dsRNAs构建了cDNA。M1和M2 cDNA序列分别由1645和1445bp组成。序列分析表明，每个dsRNA仅在一条链上有一个单一的长开放阅读框（ORF）。M1 ORF编码一个519个氨基酸残基的多肽，预测分子量为60 kDa。在预测的氨基酸序列中鉴定出依赖RNA的RNA聚合酶保守基序，并且该聚合酶在体外合成dsRNA。M2 ORF编码一个413个氨基酸残基的多肽，预测分子量为44 kDa。预测的氨基酸序列包含与在纯化的FusoV 44 kDa衣壳蛋白中发现的序列相对应的序列。
