人类巨细胞病毒的DNA结合蛋白pUL57：特异性免疫球蛋白M（IgM）反应性与针对其他主要靶抗原的IgM反应性的比较
The DNA-binding protein pUL57 of human cytomegalovirus: comparison of specific immunoglobulin M (IgM) reactivity with IgM reactivity to other major target antigens.
作者信息
Maine G T, Lazzarotto T, Chovan L E, Flanders R, Landini M P
机构信息
Abbott Laboratories, Abbott Park, Illinois, USA.
出版信息
Clin Diagn Lab Immunol. 1996 May;3(3):358-60. doi: 10.1128/cdli.3.3.358-360.1996.
Abstract
In this work we used PCR to amplify the DNA regions coding for two polypeptides from pUL57 of human cytomegalovirus (amino acids 540 to 601 and 1144 to 1233) and showed that both portions reacted very efficiently with immunoglobulin M in sera of acutely infected subjects. However, pUL57 is not an essential antigen for the replacement of or supplement to a cocktail of recombinant protein antigens containing portions of ppUL32, -44, -83, and -80a in immunoglobulin M serology.
摘要
在本研究中，我们使用聚合酶链反应（PCR）扩增了人巨细胞病毒pUL57编码两种多肽的DNA区域（氨基酸540至601以及1144至1233），并表明这两个部分在急性感染受试者血清中与免疫球蛋白M反应非常有效。然而，在免疫球蛋白M血清学中，pUL57并非替代或补充包含ppUL32、-44、-83和-80a部分的重组蛋白抗原混合物的必需抗原。
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