巨细胞病毒特异性免疫球蛋白M的检测：新型蛋白质印迹法、传统蛋白质印迹法与九种市售检测方法的比较
Search for cytomegalovirus-specific immunoglobulin M: comparison between a new western blot, conventional western blot, and nine commercially available assays.
作者信息
Lazzarotto T, Brojanac S, Maine G T, Landini M P
机构信息
Department of Clinical and Experimental Medicine, University of Bologna, Italy.
出版信息
Clin Diagn Lab Immunol. 1997 Jul;4(4):483-6. doi: 10.1128/cdli.4.4.483-486.1997.
Abstract
We tested 101 serum samples obtained from pregnant women for the presence of human cytomegalovirus (HCMV)-specific IgM with nine different commercially available kits and with two Western blotting (WB) tests previously developed in our laboratory. The conventional WB test contains viral structural proteins separated on a gel from purified HCMV particles and transferred to nitrocellulose. The new WB test contains viral structural proteins and three recombinant proteins which contain the significant immunogenic portions of pp150 (ppUL32), pp52 (ppUL44), and p130 (pUL57). The results obtained indicate that the new WB test combines high specificity (92.5%) with high sensitivity (95.0%), characteristics that, in combination, have not been obtained with any of the other tests.
摘要
我们使用9种不同的市售试剂盒以及我们实验室之前开发的两种蛋白质印迹法（WB），对从孕妇身上采集的101份血清样本进行检测，以确定是否存在人巨细胞病毒（HCMV）特异性IgM。传统的WB检测包含从纯化的HCMV颗粒中在凝胶上分离并转移至硝酸纤维素膜上的病毒结构蛋白。新的WB检测包含病毒结构蛋白以及三种重组蛋白，这三种重组蛋白包含pp150（ppUL32）、pp52（ppUL44）和p130（pUL57）的重要免疫原性部分。所得结果表明，新的WB检测结合了高特异性（92.5%）和高灵敏度（95.0%），而其他任何检测都未同时具备这两种特性。
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