Senchenkova S N, Shashkov A S, Knirel Y A, McGovern J J, Moran A P
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Eur J Biochem. 1996 Jul 15;239(2):434-8. doi: 10.1111/j.1432-1033.1996.0434u.x.
An O-specific polysaccharide was liberated from Campylobacter fetus subsp. fetus serotype B lipopolysaccharide by mild acid hydrolysis followed by gel chromatography. This polysaccharide was found to contain D-rhamnose and 3-O-methyl-D-rhamnose (D-Rha3Me, D-acofriose) in a ratio of approximately 24:1, as well as lipopolysaccharide core constituents. The structure of the polysaccharide was studied by 1H-NMR and 13C-NMR spectroscopy, which included two-dimensional COSY, rotating-frame NOE spectroscopy (ROESY), and computer-assisted analysis of the 13C-NMR spectrum. Methylation analysis using [2H3]methyl iodide and Smith degradation followed by GLC/MS of the derived acetylated oligosaccharide-alditols was used to determine the location of D-acofriose. The O-specific polysaccharide is linear, consists on average of 12 disaccharide repeating units, and is terminated by a residue of D-acofriose. The following structure of the D-rhamnan chain was established: [sequence: see text]
通过温和酸水解继以凝胶色谱法，从胎儿弯曲菌胎儿亚种B血清型脂多糖中释放出一种O-特异性多糖。发现该多糖含有比例约为24:1的D-鼠李糖和3-O-甲基-D-鼠李糖（D-Rha3Me，D-阿考弗糖），以及脂多糖核心成分。通过1H-NMR和13C-NMR光谱对该多糖的结构进行了研究，其中包括二维COSY、旋转框架NOE光谱（ROESY）以及13C-NMR光谱的计算机辅助分析。使用[2H3]甲基碘进行甲基化分析并继以Smith降解，随后对衍生的乙酰化寡糖糖醇进行GLC/MS分析，以确定D-阿考弗糖的位置。该O-特异性多糖是线性的，平均由12个二糖重复单元组成，并以一个D-阿考弗糖残基终止。确定了D-鼠李聚糖链的以下结构：[序列：见原文]
