Senchenkova S N, Shashkov A S, Knirel Y A, McGovern J J, Moran A P
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow.
Eur J Biochem. 1997 May 1;245(3):637-41. doi: 10.1111/j.1432-1033.1997.00637.x.
A polysaccharide fraction liberated from Campylobacter fetus subsp. fetus serotype A lipopolysaccharide by mild acid hydrolysis followed by gel-permeation chromatography contained a partially O-acetylated D-mannan chain, as an O-specific polysaccharide, with a core oligosaccharide attached. The structure of the polysaccharide was studied by O-deacetylation, methylation, and 1H- and 13C-NMR spectroscopy, including computer-assisted analysis of the 13C-NMR spectrum. A structure of -->3)-alpha-D-Manp2Ac-(1--> was established as the structure of the O-specific polysaccharide, the degree of O-acetylation of the mannose residues at position 2 being estimated as 80-90%. As judged by the ratio of mannose to core constituents, the D-mannan chain consists on average of 10-12 monosaccharide units.
通过温和酸水解从胎儿弯曲杆菌亚种胎儿血清型A脂多糖中释放出的多糖级分，经凝胶渗透色谱法分离后，包含一条部分O-乙酰化的D-甘露聚糖链，作为O-特异性多糖，并连接有一个核心寡糖。通过O-脱乙酰化、甲基化以及1H-和13C-NMR光谱（包括13C-NMR光谱的计算机辅助分析）对该多糖的结构进行了研究。确定了→3)-α-D-Manp2Ac-(1→的结构为O-特异性多糖的结构，2位甘露糖残基的O-乙酰化程度估计为80-90%。根据甘露糖与核心成分的比例判断，D-甘露聚糖链平均由10-12个单糖单元组成。
