Zdorovenko E L, Ovod V V, Zatonsky G V, Shashkov A S, Kocharova N A, Knirel Y A
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow.
Carbohydr Res. 2001 Feb 28;330(4):505-10. doi: 10.1016/s0008-6215(01)00005-2.
The O-methylation pattern of the O polysaccharide (OPS) of the lipopolysaccharide of Pseudomonas syringae pv. phaseolicola GSPB 1552 was revealed by methylation (CD3I) analysis, Smith degradation, and NMR spectroscopy. Together with the major O repeats consisting of D-rhamnopyranose (D-Rhap) and D-fucofuranose (D-Fucf), there are minor repeats (approximately 30%) containing 3-O-methyl-D-rhamnose (D-acofriose), which is 2-substituted in the interior repeats and occupies the terminal non-reducing end of the OPS. It was suggested that the methylated O repeats are linked to each other nearby the non-reducing end of the OPS and that the 'biological' O repeat of the OPS has the following structure: [molecular structure: see text].
通过甲基化（CD3I）分析、史密斯降解和核磁共振光谱揭示了丁香假单胞菌菜豆致病变种GSPB 1552脂多糖的O多糖（OPS）的O-甲基化模式。除了由D-鼠李吡喃糖（D-Rhap）和D-岩藻呋喃糖（D-Fucf）组成的主要O重复序列外，还有少量重复序列（约30%）含有3-O-甲基-D-鼠李糖（D-阿考弗糖），其在内部重复序列中为2-取代，并占据OPS的末端非还原端。有人提出，甲基化的O重复序列在OPS的非还原端附近相互连接，且OPS的“生物学”O重复序列具有以下结构：[分子结构：见正文]
