Kaiser U, Hofmann J, Schilli M, Wegmann B, Klotz U, Wedel S, Virmani A K, Wollmer E, Branscheid D, Gazdar A F, Havemann K
Philipps Universität Marburg, Department of Internal Medicine, Germany.
Int J Cancer. 1996 Jul 29;67(3):357-64. doi: 10.1002/(SICI)1097-0215(19960729)67:3<357::AID-IJC9>3.0.CO;2-Q.
Female gender is a significant independent favorable prognostic factor in lung cancer. To study the possible role of sex hormones in lung cancer, the expression of sex-steroid receptors and the glucocorticoid receptor was investigated in 29 lung-cancer cell lines stemming from small-cell lung cancer (SCLC) and non-small-cell lung cancer (NSCLC) by means of immunocytochemistry, ligand-binding assays and RNA expression via polymerase chain reaction. In at least 2 methods of investigation, NSCLC cell lines showed a low expression of estrogen receptor in 6, progesterone receptor in 13 and androgen receptor in 12 out of 17 cases examined; sex-steroid-receptor expression was virtually absent in SCLC cell lines. The glucocorticoid receptor was expressed in all 29 cell lines studied. Additionally, 52 tumor samples from primary lung cancer were investigated for their receptor expression by means of immunohistochemistry. Among patients with primary lung-cancer sex-steroid-receptor expression in tumor biopsies was detected most frequently in female patients (in 69% of 16 cases, vs. 42% of 36 tumors from men) and in patients with adenocarcinoma. Further research will focus on these subgroups. Immunohistology is a feasible method of studying steroid-receptor expression in lung cancer.
女性性别是肺癌重要的独立预后有利因素。为研究性激素在肺癌中的可能作用，通过免疫细胞化学、配体结合测定以及聚合酶链反应检测RNA表达，对来自小细胞肺癌（SCLC）和非小细胞肺癌（NSCLC）的29个肺癌细胞系进行了性类固醇受体和糖皮质激素受体表达的研究。在至少两种检测方法中，在17例检测的NSCLC细胞系中，6例雌激素受体、13例孕激素受体和12例雄激素受体表达较低；SCLC细胞系中几乎不存在性类固醇受体表达。所研究的29个细胞系均表达糖皮质激素受体。此外，采用免疫组织化学方法对52例原发性肺癌肿瘤样本的受体表达进行了研究。在原发性肺癌患者中，肿瘤活检中性类固醇受体表达在女性患者（16例中的69%，相比之下男性36例肿瘤中的42%）和腺癌患者中最为常见。进一步的研究将聚焦于这些亚组。免疫组织学是研究肺癌中类固醇受体表达的一种可行方法。
