Kuimova T F, Sokolov A A
Mikrobiologiia. 1977 Jan-Feb;46(1):155-60.
Ultrastructural changes were found to occur in the mycelium of Actinomyces hygroscopicus, strain 33x, which produced an exocellular proteolytic enzyme during submerged fermentation, in both laboratory and semiindustrial conditions (in 100-1 fermenters). In the course of the enzyme accumulation, numerous vacuoles appeared in the hyphae, some hyphae became wider, and the cell walls were more loose. The greatest structural changes were found in the intracytoplasmic membrane systems. The ultrastructural changes of the mycelium are not presumably connected with the accumulation of the enzyme, but are the result of the differentiation of cellular structures during aging of the cells and their transition to the stage of autolysis.
在实验室和半工业条件下（在100升发酵罐中），发现在吸水放线菌33x菌株的菌丝体中发生了超微结构变化，该菌株在深层发酵过程中产生一种胞外蛋白酶。在酶积累过程中，菌丝中出现大量液泡，一些菌丝变宽，细胞壁更疏松。在胞内膜系统中发现了最大的结构变化。菌丝体的超微结构变化大概与酶的积累无关，而是细胞老化及其向自溶阶段转变过程中细胞结构分化的结果。
