Tay F R, Gwinnett A J, Pang K M, Wei S H
Department of Children's Dentistry and Orthodontics, Faculty of Dentistry, University of Hong Kong.
J Dent Res. 1996 Apr;75(4):1034-44. doi: 10.1177/00220345960750040601.
Preservation of the morphological integrity of demineralized dentin collagen in its hydrated state may account for the success observed in wet-bonding procedures. This study investigated the micromorphological differences between moist- and dry-bonding techniques with the use of: (a) Aelitebond, an alcohol-based, water-free, single-component dentin adhesive primer system; and (b) a water-free, acetone-based experimental primer similar to the acetone-based, water-containing All-Bond 2, a two-component primer system. In the wet groups, acid-conditioned dentin surfaces were blotted so that they remained visibly moist prior to bonding. In the dry groups, dentin surfaces were air-dried for 30 sec. Following the bonding procedures, dentin discs in each group were laminated together by means of a chemical-cure resin and processed for scanning electron microscope (SEM) and transmission electron microscope (TEM) examination. Conditioning with 10% H3PO4 for 20 sec. produced complete demineralization of the outer dentin. In the wet groups, banded collagen and interfibrillar spaces could be observed at the surface of the acid-conditioned dentin. Complete wetting of the loosely arranged collagen fibrils by the resin resulted in the formation of a hybrid layer. In the dry groups, only a very thin hybrid layer was observed on the dentin surface, along the walls of the tubules, and along the course of their lateral branches. The absence of banded collagen and interfibrillar spaces within these areas suggested the existence of a collapsed dentin matrix along various liquid-vapor boundaries that restricted resin permeation into the subsurface intertubular matrix, producing an incompletely infiltrated "hybridoid region".
脱矿牙本质胶原在其水合状态下形态完整性的保持可能是湿粘结程序取得成功的原因。本研究使用以下材料调查了湿粘结技术和干粘结技术之间的微观形态差异：(a) Aelitebond，一种基于酒精的、无水的单组分牙本质粘结剂底漆系统；(b) 一种类似于基于丙酮、含水的双组分底漆系统All-Bond 2的无水、基于丙酮的实验性底漆。在湿粘结组中，酸处理过的牙本质表面被吸干，以便在粘结前保持明显湿润。在干粘结组中，牙本质表面风干30秒。粘结程序完成后，每组中的牙本质圆盘通过化学固化树脂层压在一起，并进行扫描电子显微镜（SEM）和透射电子显微镜（TEM）检查。用10% H3PO4处理20秒可使外层牙本质完全脱矿。在湿粘结组中，在酸处理过的牙本质表面可观察到带状胶原和纤维间间隙。树脂对松散排列的胶原纤维的完全湿润导致了混合层的形成。在干粘结组中，仅在牙本质表面、沿着小管壁及其侧支的走行观察到非常薄的混合层。这些区域内不存在带状胶原和纤维间间隙，这表明沿着各种液-气边界存在塌陷的牙本质基质，限制了树脂渗透到牙本质小管间的基质中，从而产生了不完全浸润的“类混合区域”。
