Tay F R, Gwinnett A J, Wei S H
Department of Children's Dentistry and Orthodontics, Faculty of Dentistry, University of Hong Kong.
Am J Dent. 1996 Jun;9(3):109-14.
To study with the scanning electron microscope the interfacial phenomenon that occurred in the presence of excessive surface moisture following the application of an acetone-based adhesive to acid-conditioned dentin.
Specimens consisted of flat dentin discs that were divided into three groups based upon the status of remaining surface moisture: (1) 3-second dry group, (2) blot-dry group, with the conditioned dentin surface remaining shiny as opposed to matte, and (3) overwet group: an additional 40 mL of distilled water spread thin on the dentin surface after blot-drying. Polished resin-dentin interfaces were brought into relief using plasma-etching and examined without further embedding.
Although a hybrid layer was observed in all three groups, there was (1) a sequential deterioration of the bonded assembly along the hybrid layer-resin interface, and (2) loss of complete tubular seal as the quantity of surface moisture increased. Intratubular resin globules were observed beneath short resin plugs in the 3-second dry group. Small, isolated blister-like spaces, trapped within the resin layer, were observed along the surface of the hybrid layer in the blot-dry group, while similar, but larger spaces were found in the overwet group. The blister-like spaces, which were often continuous with incompletely sealed tubular orifices, were partially filled with extraneous resin globules dispersed within an amorphous matrix.
使用扫描电子显微镜研究在酸蚀处理后的牙本质上涂抹丙酮基粘合剂后，在存在过多表面水分的情况下发生的界面现象。
标本由扁平的牙本质圆盘组成，根据剩余表面水分的状况分为三组：（1）3秒干燥组，（2）吸干干燥组，与哑光状态相反，酸蚀处理后的牙本质表面保持光亮，（3）过度湿润组：吸干干燥后在牙本质表面再薄薄地铺展40 mL蒸馏水。使用等离子蚀刻使抛光的树脂-牙本质界面凸显出来，无需进一步包埋即可进行检查。
尽管在所有三组中均观察到混合层，但存在以下情况：（1）沿着混合层-树脂界面的粘结组件逐渐恶化，（2）随着表面水分量的增加，完全的管状封闭丧失。在3秒干燥组的短树脂栓下方观察到管内树脂小球。在吸干干燥组中，沿着混合层表面观察到被困在树脂层内的小的、孤立的水泡样间隙，而在过度湿润组中发现了类似但更大的间隙。水泡样间隙通常与未完全封闭的管孔相连，部分填充有分散在无定形基质中的外来树脂小球。
