Whitham S, McCormick S, Baker B
Department of Plant Biology, University of California, Berkeley 94720, USA.
Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8776-81. doi: 10.1073/pnas.93.16.8776.
It has been proposed that cloned plant disease resistance genes could be transferred from resistant to susceptible plant species to control important crop plant diseases. The recently cloned N gene of tobacco confers resistance to the viral pathogen, tobacco mosaic virus. We generated transgenic tomato plants bearing the N gene and demonstrate that N confers a hypersensitive response and effectively localizes tobacco mosaic virus to sites of inoculation in transgenic tomato, as it does in tobacco. The ability to reconstruct the N-mediated resistance response to tobacco mosaic virus in tomato demonstrates the utility of using isolated resistance genes to protect crop plants from diseases, and it demonstrates that all the components necessary for N-mediated resistance are conserved in tomato.
有人提出，可以将克隆的植物抗病基因从抗病植物物种转移到感病植物物种，以控制重要的农作物病害。最近克隆的烟草N基因赋予了对病毒病原体烟草花叶病毒的抗性。我们培育了携带N基因的转基因番茄植株，并证明N基因在转基因番茄中能引发超敏反应，并有效地将烟草花叶病毒限制在接种部位，就像在烟草中一样。在番茄中重建N介导的对烟草花叶病毒的抗性反应的能力，证明了使用分离的抗性基因保护农作物免受病害的实用性，也证明了N介导的抗性所需的所有成分在番茄中是保守的。
