Topper M J, Prasse K W
Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens 30602, USA.
Am J Vet Res. 1996 Apr;57(4):456-62.
To evaluate new ELISA for measurement of thrombin-antithrombin III (TAT) concentration, and to correlate the values to other tests of hemostasis in horses with colic.
Plasma TAT concentration and 8 other hemostasis analytes were measured in horses with colic at hospital admission and during the next 4 days. Retrospectively, data were analyzed by outcome, broad-category diagnosis, and clinical management, and for correlation between TAT and other assays.
100 horses with colic.
Plasma samples were evaluated for TAT, fibrinogen, and fibrin degradation products concentrations; antithrombin III (ATIII), protein C, alpha 2-antiplasmin, and plasminogen activities; prothrombin time (PT); and activated partial thromboplastin time.
Changes were indicative of a hypercoagulable state, most severe in nonsurviving horses, characterized by increased TAT concentration; decreased ATIII, protein C, and plasminogen activities; and increased PT. Nonsurvivors had significantly increased TAT concentration compared with that in survivors, without regard to sample collection time; however, compared over time, TAT was significantly increased only at admission. Highest TAT concentration was in nonsurvivors with inflammatory intestinal lesions. There was significant negative correlation between TAT and ATIII, protein C, alpha 2-antiplasmin, and plasminogen values, and significant positive correlation between TAT and PT, and fibrin degradation products values.
Plasma TAT reflects the current state of coagulation system activation and is a good assay for early diagnosis of the hypercoagulable state in horses with the most severe forms of colic.
Measurement of equine TAT provides further information to characterize the hypercoagulable state in horses to aid in case management.
评估用于测量凝血酶 - 抗凝血酶III（TAT）浓度的新型酶联免疫吸附测定（ELISA），并将该值与患急腹症马匹的其他止血测试结果相关联。
在患急腹症的马匹入院时及随后4天内测量血浆TAT浓度和其他8种止血分析物。回顾性地，通过结果、广泛分类诊断和临床管理对数据进行分析，并分析TAT与其他检测之间的相关性。
100匹患急腹症的马。
评估血浆样本中的TAT、纤维蛋白原和纤维蛋白降解产物浓度；抗凝血酶III（ATIII）、蛋白C、α2 - 抗纤溶酶和纤溶酶原活性；凝血酶原时间（PT）；以及活化部分凝血活酶时间。
变化表明存在高凝状态，在未存活的马匹中最为严重，其特征为TAT浓度升高；ATIII、蛋白C和纤溶酶原活性降低；以及PT升高。无论样本采集时间如何，未存活马匹的TAT浓度均显著高于存活马匹；然而，随着时间推移进行比较，TAT仅在入院时显著升高。TAT浓度最高的是患有炎症性肠道病变的未存活马匹。TAT与ATIII、蛋白C、α2 - 抗纤溶酶和纤溶酶原值之间存在显著负相关，TAT与PT以及纤维蛋白降解产物值之间存在显著正相关。
血浆TAT反映了凝血系统激活的当前状态，是早期诊断最严重形式急腹症马匹高凝状态的良好检测方法。
测量马的TAT可提供更多信息来表征马的高凝状态，以辅助病例管理。
