Entrala E, Rueda-Rubio M, Janssen D, Mascaró C
Instituto de Biotecnología, Departmento de Parasitologia, Facultad de Ciencias, Polígono Universitario de Fuentenueva s/n, Granada, Spain.
Int J Parasitol. 1995 Dec;25(12):1473-7. doi: 10.1016/0020-7519(95)00091-7.
Infections by the protozoan parasite Cryptosporidium parvum are routinely diagnosed by modified Ziehl-Neelsen (acid-fast) staining of faecal preparations despite the counterstaining and ghost-like appearance of some oocysts. Quantitative studies demonstrated that only a small percentage of oocysts excreted by naturally infected newborn calves displayed acid-fast characteristics, but that percentage increased when the time between excretion and sample staining was increased. The treatment of faecal samples with hydrogen peroxide (10 min, 5 vol. final concentration) caused all oocysts to become acid-fast, with up to 40-fold increases in test sensitivity in samples treated and stained within 3 h of excretion. Flow-cytometry analysis of hydrogen peroxide-treated oocysts also demonstrated increased labelling of oocysts by a commercial monoclonal antibody preparation commonly used for diagnosis.
尽管粪便标本中有些隐孢子虫卵囊存在复染和幽灵般的外观，但微小隐孢子虫的感染通常仍通过改良齐-尼（抗酸）染色法来诊断。定量研究表明，自然感染的新生犊牛排出的卵囊中只有一小部分具有抗酸特性，但当排出时间与样本染色时间间隔增加时，这一比例会升高。用过氧化氢处理粪便样本（10分钟，最终浓度5体积）会使所有卵囊都具有抗酸特性，在排出后3小时内处理并染色的样本中，检测灵敏度提高了40倍。用过氧化氢处理后的卵囊进行流式细胞术分析也表明，一种常用于诊断的商业单克隆抗体制剂对卵囊的标记有所增加。
