Carrozzo M, Broccoletti R, Carbone M, Gandolfo S, Garzino P, Cascio G
Department of Oral Medicine and Periodontology, School of Medicine and Dentistry, University of Turin, Italy.
Bull Group Int Rech Sci Stomatol Odontol. 1996 Feb-Mar;39(1-2):33-8.
Quantitative analysis of peripheral blood lymphocytes was carried out in 25 patients with atrophic-erosive type of oral lichen planus (OLP) (Group 1), in 28 patients with reticular-plaque like lesions of OLP (Group 2) and in 21 healthy patients (Group 3) by using flow cytometry. CD4+ subsets decreased significantly in patients with reticular-plaque like varieties when compared with healthy patients (Group 3) (One way analysis of variance p = 0.039; t-test with Bonferroni correction p < 0.05). Moreover, in patients with hyperkeratosic forms of OLP (Group 2) CD8+ cell populations were significantly higher than in controls (Group 3) (Kruskal-Wallis test p = 0.035; Mann-Whitney test with Bonferroni's correction p < 0.0001) and consequently CD4/CD8 ratio was significantly lower in patients with reticular-plaque like lesions than in controls (Kruskal-Wallis test p = 0.01; Mann-Whitney test with Bonferroni's correction p = 0.013). No statistical differences between patients of Group 1 (atrophic-erosive OLP) and the other two Groups (hyperkeratosic OLP and healthy controls) were detected. 40% of the patients of Group 1 were affected by chronic hepatopathies, most of which were related to hepatitis C virus (HCV), but the data were not substantially modified after adjustment for the patients with chronic liver disease HCV positive. There is no clear evidence that these results indicate the existence of a different pathogenetic mechanism between erosive-atrophic and hyperkeratosic types of OLP. On the other hand, these results and the previously reported immunohistochemical findings suggest that quantitative alterations of peripheral blood lymphocytes in hyperkeratosic varieties of OLP could represent a shift of CD4+ cells from the vascular to the oral mucosa compartment.
采用流式细胞术对25例萎缩糜烂型口腔扁平苔藓（OLP）患者（第1组）、28例网状斑块样OLP病变患者（第2组）和21例健康患者（第3组）的外周血淋巴细胞进行了定量分析。与健康患者（第3组）相比，网状斑块样病变患者的CD4 +亚群显著减少（单因素方差分析p = 0.039；Bonferroni校正t检验p < 0.05）。此外，OLP角化过度型患者（第2组）的CD8 +细胞群体显著高于对照组（第3组）（Kruskal-Wallis检验p = 0.035；Bonferroni校正Mann-Whitney检验p < 0.0001），因此，网状斑块样病变患者的CD4/CD8比值显著低于对照组（Kruskal-Wallis检验p = 0.01；Bonferroni校正Mann-Whitney检验p = 0.013）。未检测到第1组患者（萎缩糜烂型OLP）与其他两组患者（角化过度型OLP和健康对照）之间的统计学差异。第1组40%的患者患有慢性肝病，其中大多数与丙型肝炎病毒（HCV）有关，但在对HCV阳性的慢性肝病患者进行校正后，数据没有实质性改变。没有明确证据表明这些结果表明糜烂萎缩型和角化过度型OLP之间存在不同的发病机制。另一方面，这些结果和先前报道的免疫组化结果表明，OLP角化过度型外周血淋巴细胞的定量改变可能代表CD4 +细胞从血管向口腔黏膜腔室的转移。
