Proudman C J, Trees A J
Liverpool School of Tropical Medicine/Faculty of Veterinary Science, University of Liverpool, UK.
Vet Parasitol. 1996 Feb;61(3-4):239-47. doi: 10.1016/0304-4017(95)00837-3.
Whole worm extract (WWE) and excretory/secretory (E/S) antigens of Anoplocephala perfoliata were characterised by SDS-PAGE and their use in the serodiagnosis of equine cestodosis was evaluated. An enzyme-linked immunosorbent assay (ELISA) was used to compare WWE and E/S antigen as the capture layer in an antibody capture ELISA. E/S antigen gave the best differentiation between sera from tapeworm-positive and tapeworm-negative horses. The E/S-ELISA was optimised and validated against sera from horses of known tapeworm status. This assay gave a diagnostic sensitivity of 68% (n = 38) and a specificity in helminth-naive horses of 95% (n = 20). Western blot analysis of tapeworm-positive and tapeworm-negative sera revealed the immunoreactive proteins of E/S antigen to be those of 12 and 13 kDa.
采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）对叶状裸头绦虫的全虫提取物（WWE）和排泄/分泌（E/S）抗原进行了表征，并评估了它们在马绦虫病血清诊断中的应用。在抗体捕获酶联免疫吸附测定（ELISA）中，使用酶联免疫吸附测定法比较了WWE和E/S抗原作为捕获层的情况。E/S抗原在区分绦虫阳性和绦虫阴性马匹的血清方面表现最佳。对E/S-ELISA进行了优化，并针对已知绦虫感染状况的马匹血清进行了验证。该检测方法的诊断敏感性为68%（n = 38），在未感染蠕虫的马匹中的特异性为95%（n = 20）。对绦虫阳性和绦虫阴性血清的蛋白质印迹分析显示，E/S抗原的免疫反应性蛋白为12和13 kDa的蛋白质。
