Slater Rachael, Frau Alessandra, Hodgkinson Jane, Archer Debra, Probert Chris
Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Crown Street, Liverpool L69 3GE, UK.
Institute of Infection, Veterinary and Ecological Science, University of Liverpool, Leahurst Campus, Chester High Road, Wirral CH64 7TE, UK.
Animals (Basel). 2021 Mar 9;11(3):755. doi: 10.3390/ani11030755.
is a common equine tapeworm associated with an increased risk of colic (abdominal pain) in horses. Identification of parasite and intestinal microbiota interactions have consequences for understanding the mechanisms behind parasite-associated colic and potential new methods for parasite control. was diagnosed by counting of worms in the caecum post-mortem. Bacterial DNA was extracted from colonic contents and sequenced targeting of the 16S rRNA gene (V4 region). The volatile organic compound (VOC) metabolome of colonic contents was characterised using gas chromatography mass spectrometry. Bacterial diversity (alpha and beta) was similar between tapeworm infected and non-infected controls. Some compositional differences were apparent with down-regulation of operational taxonomic units (OTUs) belonging to the symbiotic families of Ruminococcaceae and Lachnospiraceae in the tapeworm-infected group. Overall tapeworm burden accounted for 7-8% of variation in the VOC profile (permutational multivariate analysis of variance). Integration of bacterial OTUs and VOCs demonstrated moderate to strong correlations indicating the potential of VOCs as markers for bacterial OTUs in equine colonic contents. This study has shown potential differences in the intestinal microbiome and metabolome of infected and non-infected horses. This pilot study did not control for extrinsic factors including diet, disease history and stage of infection.
是一种常见的马绦虫，与马患绞痛（腹痛）的风险增加有关。寄生虫与肠道微生物群相互作用的识别对于理解寄生虫相关性绞痛背后的机制以及寄生虫控制的潜在新方法具有重要意义。通过在死后对盲肠中的蠕虫进行计数来诊断。从结肠内容物中提取细菌DNA，并针对16S rRNA基因（V4区域）进行测序。使用气相色谱质谱法对结肠内容物的挥发性有机化合物（VOC）代谢组进行表征。绦虫感染组和未感染对照组之间的细菌多样性（α和β）相似。在绦虫感染组中，属于共生科瘤胃球菌科和毛螺菌科的操作分类单元（OTU）下调，一些组成差异明显。绦虫总体负担占VOC谱变异的7-8%（置换多变量方差分析）。细菌OTU和VOC的整合显示出中度到强的相关性，表明VOC作为马结肠内容物中细菌OTU标记物的潜力。这项研究表明感染和未感染马的肠道微生物组和代谢组存在潜在差异。这项初步研究没有控制包括饮食、疾病史和感染阶段在内的外部因素。
