[Characterization of chick embryo fibroblast culture--overview of its effectiveness as a hybridoma cell].

The use of culture supernatant (added in a final concentration of 25% v/v) from chicken embryonal fibroblasts (CEF) for stimulation of established hybridoma cell lines has been shown. The proliferative effect on hybridoma cells from different origins was heatproof and was possible with a reduced serum part (FCS) in the culture medium. After a chromatografic separation the supernatant sections were tested and the positive effect was much lower in comparison to the total conditioned media.