Sávoly S B, Kovár J, Rossow N
Institute of Drug Research, Academy of Sciences of the GDR, Berlin.
Acta Histochem Suppl. 1990;39:125-9.
After stepwise adaptation to PFH-medium with low serum levels over a period of 4 weeks two pools of the IWF-F2 hybridoma have been obtained which can be grown continuously in PFH-medium supplemented with 2 resp. 1% of bovine serum without loss of production of monoclonal antibody. Adaptation of this hybridoma to growth without any serum supplement was not possible during the course of this experimental series.
在4周的时间内逐步适应低血清水平的PFH培养基后，获得了两批IWF-F2杂交瘤细胞株，它们可以在添加了2%和1%牛血清的PFH培养基中连续培养，而不会丧失单克隆抗体的产生能力。在本实验系列过程中，无法使这种杂交瘤适应无任何血清补充剂的生长条件。
