Proliferation of hybridoma cells in low-serum medium.

After stepwise adaptation to PFH-medium with low serum levels over a period of 4 weeks two pools of the IWF-F2 hybridoma have been obtained which can be grown continuously in PFH-medium supplemented with 2 resp. 1% of bovine serum without loss of production of monoclonal antibody. Adaptation of this hybridoma to growth without any serum supplement was not possible during the course of this experimental series.