Müller T, Henning K, Kramer M, Czerny C P, Meyer H, Ziedler K
Institut für Epidemiologische Diagnostik, Bundesforschungsanstalt für Viruskrankheiten der Tiere Wusterhausen, Germany.
J Wildl Dis. 1996 Apr;32(2):348-53. doi: 10.7589/0090-3558-32.2.348.
The prevalence of orthopox virus (OPV)-specific antibodies in 1,040 red foxes (Vulpes vulpes) was evaluated on a large scale in the German Federal State Brandenburg. Serum samples were selected from 809 communities within the study area from January 1991 to September 1994 by simple random sampling. Screening was carried out by an indirect enzyme-linked immunosorbent assay (ELISA). Orthopox virus-specific antibodies were found in 162 (16%) of the 1,040 fox sera. Furthermore 154 (15%) sera were considered suspect positive. The specificity of the antibodies detected in ELISA-positive and suspect positive sera was confirmed by Western blotting. Presence of OPV-antibodies occurred in 291 communities. No correlation of OPV-antibodies findings to latitude or characteristic topographical and ecological peculiarities of the study area was found. Although the causative agent is still unknown we believe that orthopox viruses probably have a ubiquitous presence among red foxes.
在德国勃兰登堡州，对1040只赤狐（赤狐属）中的正痘病毒（OPV）特异性抗体流行情况进行了大规模评估。1991年1月至1994年9月期间，通过简单随机抽样从研究区域内的809个群落中选取血清样本。采用间接酶联免疫吸附测定（ELISA）进行筛查。在1040份狐狸血清中，有162份（16%）检测出正痘病毒特异性抗体。此外，154份（15%）血清被视为疑似阳性。通过蛋白质印迹法确认了ELISA阳性和疑似阳性血清中检测到的抗体的特异性。在291个群落中发现了OPV抗体。未发现OPV抗体的检测结果与研究区域的纬度或典型地形及生态特征之间存在相关性。尽管病原体仍不明，但我们认为正痘病毒可能在赤狐中普遍存在。
