Wurm R, Dobler G, Peters M, Kiessig S T
Staatliches Veterinäruntersuchungsamt, Arnsberg, Germany.
J Vet Med B Infect Dis Vet Public Health. 2000 Sep;47(7):503-9. doi: 10.1046/j.1439-0450.2000.00373.x.
Serum samples from 786 red foxes shot between January 1995 and August 1996 in the southern half of Northrhine-Westphalia, located in western Germany, were tested for the presence of antibodies against tick-borne encephalitis (TBE) virus using the Immunozym FSME IgG All Species-ELISA (Immuno, Heidelberg, Germany) as a screening test: 759 sera were negative, 23 (2.9%) were borderline, and four (0.5%) were positive. Nine of the 27 ELISA reactive sera were confirmed by the TBE Western-Blot (Immuno, Heidelberg, Germany). Furthermore these 27 sera were tested for neutralizing antibodies by means of a plaque reduction neutralization test (PRNT) against TBE and West Nile viruses. Only one single serum was found to have a neutralization titre (+1:800 PRNT80) against TBE virus. All other 26 sera were negative for neutralizing antibodies against TBE or West Nile virus. Since the titre of the single serum is low, it can be interpreted that if TBE virus is present, its prevalence is extremely low. Northrhine-Westphalia is not classified as a TBE-endemic area. Further calculated serological testing of game and virological investigation of collected ticks in the affected area seem to be meaningful and necessary.
对1995年1月至1996年8月间在德国西部北莱茵 - 威斯特法伦州南部射杀的786只赤狐的血清样本，使用免疫酶法FSME IgG全物种酶联免疫吸附测定（Immuno，德国海德堡）作为筛查试验，检测抗蜱传脑炎（TBE）病毒抗体的存在情况：759份血清为阴性，23份（2.9%）为临界值，4份（0.5%）为阳性。27份酶联免疫吸附测定反应性血清中的9份通过TBE免疫印迹法（Immuno，德国海德堡）得到确认。此外，通过空斑减少中和试验（PRNT）对这27份血清检测针对TBE和西尼罗河病毒的中和抗体。仅发现一份血清对TBE病毒有中和滴度（+1:800 PRNT80）。所有其他26份血清针对TBE或西尼罗河病毒的中和抗体均为阴性。由于单份血清的滴度较低，可以推断，如果存在TBE病毒，其流行率极低。北莱茵 - 威斯特法伦州未被列为TBE流行地区。对受影响地区的猎物进行进一步的血清学检测计算以及对采集的蜱进行病毒学调查似乎是有意义且必要的。
