Henning K, Czerny C P, Meyer H, Müller T, Kramer M
Federal Research Center for Virus Diseases of Animals, Wusterhausen/Dosse, Germany.
Vet Microbiol. 1995 Feb;43(2-3):251-9. doi: 10.1016/0378-1135(94)00097-g.
703 blood samples from red foxes (Vulpes vulpes) were investigated to determine the prevalence of antibody against an orthopox virus (vaccinia virus strain Elstree). A blocking-ELISA based on a neutralizing monoclonal antibody was used. In this assay 46 sera (6.5%) were positive with titers of 1:2 to 1:16. ELISA-results were confirmed by the plaque reduction test with 44 of the 46 sera reacting positively. The specificity of antibodies in 21 selected sera was also demonstrated by Western blot analysis.
对703份赤狐（赤狐属）血样进行了检测，以确定抗正痘病毒（痘苗病毒株埃尔斯特里）抗体的流行情况。使用了基于中和单克隆抗体的阻断ELISA法。在该检测中，46份血清（6.5%）呈阳性，效价为1:2至1:16。46份血清中的44份通过蚀斑减少试验证实ELISA结果呈阳性。21份选定血清中抗体的特异性也通过蛋白质印迹分析得以证实。
