Villar M, Lefèvre F, Bradshaw H D, Teissier du Cros E
Institut National de la Recherche Agronomique, Station d'Amélioration des Arbres Forestiers, Ardon, France.
Genetics. 1996 May;143(1):531-6. doi: 10.1093/genetics/143.1.531.
With random amplified polymorphic DNA (RAPD) markers, we have tagged a genomic region in Populus sp. involved in qualitative resistance to Melampsora larici-populina. Our approach was based on three steps: use of RAPD markers that can be quickly and efficiently researched: application of "bulked segregant analysis" technique on individuals of one interspecific family P. trichocarpa x P. deltoides to search for RAPD markers linked to resistance; and validation of these markers in two other families linked with the first one in a 2 x 2 factorial mating design. Of five detected markers, only one marker M03/04_480 was polymorphic in the three segregating families, involving 89 individuals and four different parents. We have estimated the recombination value of 1 cM with 1 cM sampling error.
利用随机扩增多态性DNA（RAPD）标记，我们在杨树中标记了一个与对落叶松杨栅锈菌定性抗性相关的基因组区域。我们的方法基于三个步骤：使用可快速有效研究的RAPD标记；对一个种间杂交组合毛果杨×美洲黑杨的个体应用“混合分组分析法”来寻找与抗性相关的RAPD标记；以及在另外两个与第一个组合通过2×2析因交配设计相连的组合中对这些标记进行验证。在检测到的五个标记中，只有一个标记M03/04_480在三个分离群体中表现出多态性，涉及89个个体和四个不同的亲本。我们估计了重组值为1厘摩，抽样误差为1厘摩。
