Oguchi H, Torii S
Department of Plastic and Reconstructive Surgery, Nagoya University School of Medicine, Japan.
Ann Plast Surg. 1996 Jan;36(1):60-4. doi: 10.1097/00000637-199601000-00012.
Thirty PTFE prostheses (internal diameter, 1.0 mm; length, 5.0 mm; fibril length, 30 microns) were implanted into rats' femoral veins by means of a coupling device (3M precise microvascular anastomosis system, 3M, St Paul, MN) and evaluated with electron microscopy at regular intervals from 1 day to 3 weeks after implantation to study, in detail, the healing process. Eighty-three percent of mechanically anastomosed grafts were found to be patent. At 1 and 3 days after implantation, the whole length of PTFE was covered with a clot layer containing platelets and a fibrin network. After 1 week, endothelial-like cells originating from the anastomotic sides grew in across the anastomoses. At 3 weeks, the prostheses were completely covered by an endothelial-like cell layer. These results demonstrate that the degree of neo-endothelialization in the microvenous PTFE prosthesis anastomosed with 3M rings was not delayed, as was seen in the healing of microarterial PTFE tubing that was mechanically anastomosed.
使用连接装置（3M精密微血管吻合系统，3M公司，明尼苏达州圣保罗市）将30个聚四氟乙烯（PTFE）假体（内径1.0毫米；长度5.0毫米；纤维长度30微米）植入大鼠股静脉，并在植入后1天至3周定期用电子显微镜进行评估，以详细研究愈合过程。结果发现，83%的机械吻合移植物保持通畅。植入后1天和3天，PTFE的全长被含有血小板和纤维蛋白网络的凝块层覆盖。1周后，源自吻合口两侧的内皮样细胞穿过吻合口生长。3周时，假体完全被内皮样细胞层覆盖。这些结果表明，与3M环吻合的微静脉PTFE假体的新生内膜化程度没有延迟，而机械吻合的微动脉PTFE导管愈合时出现了延迟现象。
