Gentile V, Modesti A, La Pera G, Vasaturo F, Modica A, Prigiotti G, Di Silverio F, Scarpa S
Urology Department, University of Rome La Sapienza, Italy.
J Androl. 1996 Mar-Apr;17(2):96-103.
The tunica albuginea and corpus cavernosum from patients with Peyronie's disease (PD), patients with veno-occlusive dysfunction (VOD), and those from normal control subjects were studied by transmission electron microscopy and immunohistochemical staining for type I, III, and V collagens, platelet-derived growth factor (PDGF) AA and BB homodimers, and PDGF alpha and beta receptors. Ultrastructural modifications resembling a fibrotic reaction were detected in the two pathological tunica albuginea, but not in those from control subjects. Ultrastructural data demonstrated a general increase in fibrous and amorphous extracellular matrix material in the pathological tunica albuginea. The amorphous material probably represents glycoproteins and proteoglycans. The fibrous material, representing collagen, appears disorganized in the tissue and does not display the typical and homogeneous diameter, size, and spatial arrangement. Large areas of extracellular and intracytoplasmic, partially degraded, fibers are visible. An increased type I/III collagen ratio was detected by immunohistochemistry in the two pathological tunica albuginea. Moreover, a strong expression of type V collagen, correlated to fibroblasts, was revealed. Fibroblasts from control tissues, on the other hand, were totally negative. Finally, PDGF AA and BB were positive in fibroblasts from pathological tunica albuginea but were negative in control tissues. PDGF beta receptor was positive in pathological and normal tissue fibroblasts. Tunica albuginea from PD and VOD show similar ultrastructural and immunohistochemical alterations, whereas the corpus cavemosum shows no visible modifications.
通过透射电子显微镜以及针对I型、III型和V型胶原蛋白、血小板衍生生长因子（PDGF）AA和BB同二聚体以及PDGFα和β受体的免疫组织化学染色，对佩罗尼氏病（PD）患者、静脉闭塞功能障碍（VOD）患者以及正常对照者的白膜和海绵体进行了研究。在两种病理性白膜中检测到了类似纤维化反应的超微结构改变，但在对照者的白膜中未检测到。超微结构数据表明，病理性白膜中纤维状和无定形细胞外基质物质普遍增加。无定形物质可能代表糖蛋白和蛋白聚糖。代表胶原蛋白的纤维状物质在组织中显得杂乱无章，不具有典型且均匀的直径、大小和空间排列。可见大片细胞外和细胞内部分降解的纤维。通过免疫组织化学检测发现，两种病理性白膜中的I/III型胶原蛋白比例增加。此外，还发现与成纤维细胞相关的V型胶原蛋白有强烈表达。另一方面，对照组织中的成纤维细胞完全呈阴性。最后，病理性白膜中的成纤维细胞中PDGF AA和BB呈阳性，但对照组织中呈阴性。PDGFβ受体在病理性和正常组织的成纤维细胞中均呈阳性。PD和VOD患者的白膜显示出相似的超微结构和免疫组织化学改变，而海绵体未显示出明显变化。
