Viora M, De Luca A, D'Ambrosio A, Antinori A, Ortona E
Department of Immunology, Istituto Superiore di Sanità, Rome, Italy.
Antimicrob Agents Chemother. 1996 May;40(5):1294-7. doi: 10.1128/AAC.40.5.1294.
The anti-Pneumocystis carinii drug effects on mitogen-, antigen-, and interleukin-2-induced proliferative responses and on natural killer (NK) cell-mediated activity were analyzed in vivo (rats) and in vitro (normal human peripheral blood mononuclear cells). Splenocytes derived from in vivo piritrexim- and clindamycin-treated rats showed a significant inhibition of mitogen-induced proliferative responses. In vitro exposure to clindamycin, piritrexim, and pyrimethamine caused an inhibition of human T lymphocyte proliferation in response to mitogen, antigen, and interleukin-2 stimulation. Rat NK cell-mediated cytotoxic activity was not affected by the drugs, and human NK cell activity was reduced only at the highest concentration (10 micrograms/ml) of the drugs. The potential immunotoxicity of the long-term administration of these agents in humans needs further investigation.
在体内（大鼠）和体外（正常人外周血单个核细胞）分析了抗卡氏肺孢子虫药物对有丝分裂原、抗原和白细胞介素-2诱导的增殖反应以及对自然杀伤（NK）细胞介导活性的影响。来自体内接受喷他脒和克林霉素治疗的大鼠的脾细胞显示有丝分裂原诱导的增殖反应受到显著抑制。体外暴露于克林霉素、喷他脒和乙胺嘧啶会抑制人T淋巴细胞对有丝分裂原、抗原和白细胞介素-2刺激的增殖反应。大鼠NK细胞介导的细胞毒性活性不受这些药物影响，而人NK细胞活性仅在药物最高浓度（10微克/毫升）时降低。这些药物在人体内长期给药的潜在免疫毒性需要进一步研究。
