Taylor K, Hornigold N, Conway D, Williams D, Ulinowski Z, Agochiya M, Fattorini P, de Jong P, Little P F, Wolfe J
Galton Laboratory, University College London, UK.
Genome Res. 1996 Apr;6(4):235-48. doi: 10.1101/gr.6.4.235.
We have used Y-specific cosmid clones in a random fingerprinting approach to build contigs on the human Y chromosome. Clones derived from two libraries have been analyzed. The construction of one library is described here, the second was the Y chromosome-specific library LLOYNCO3 "M" (Lawrence Livermore National Laboratory). To date, we have fingerprinted 4430 cosmids: 377 contigs have been constructed containing from 2 to 39 clones. Along with the singletons, we estimate that we have covered 72.5% of the euchomatic portion of the Y chromosome with fingerprinted clones. Sequence tagged sites are being used to anchor cosmids and contigs onto the YAC framework.
我们采用Y特异性黏粒克隆，通过随机指纹图谱法在人类Y染色体上构建重叠群。已对来自两个文库的克隆进行了分析。这里描述了其中一个文库的构建过程，另一个是Y染色体特异性文库LLOYNCO3 “M”（劳伦斯利弗莫尔国家实验室）。到目前为止，我们已对4430个黏粒进行了指纹图谱分析：构建了377个重叠群，每个重叠群包含2至39个克隆。连同单一克隆，我们估计已用带指纹图谱的克隆覆盖了Y染色体常染色质部分的72.5%。序列标签位点正被用于将黏粒和重叠群锚定到YAC框架上。
